A simple, sensitive, accurate and rapid reverse phase HPLC method is developed for the determination of aspirin and esomeprazole in bulk and in synthetic mixture. Chromatography was carried out on C18 column (250 mm×4.6mm×5µm particle size, make: kromasil 1005) using a mixture of 0.05% tetrahydrofuran and methanol and water in the ratio (70:30 v/v) as a mobile phase at a flow rate of 1.0 ml/min. Detection was carried out at 237 nm. The retention time of the drug aspirin and esomeprazole was 1.82 and 4.90 min. The method produced linear responses in the concentration range of 20-120 µg/ml and 5-30 µg/ml of aspirin and esomeprazole respectively. The LOD values for HPLC method for aspirin and esomeprazole were found to be 1.9585 µg/ml and 0.1181 µg/ml. The LOQ values for aspirin and esomeprazole were found to be 5.9348 µg/ml and 0.3597 µg/ml respectively. The proposed method can be used for the estimation of these drugs in bulk and in synthetic mixture.
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